Title: Production of Nanoplastics and Bioavailable Carbons by Wastewater Comamonas

Abstract: Comamonadacae bacteria are enriched on polyethylene terephthalate (PET) microplastics in wastewaters and urban rivers, but the PET-degrading mechanisms remain unclear. Here, we investigated these mechanisms with Comamonas testosteroni KF-1, a wastewater isolate, by combining microscopy, spectroscopy, proteomics, protein modeling, and genetic engineering. Compared to minor dents on PET films, scanning electron microscopy revealed significant fragmentation of PET pellets, resulting in 3.5-fold increase in the abundance of small nanoparticles (< 100 nm) during 30-day cultivation. Infrared spectroscopy captured primarily hydrolytic cleavage in the fragmented pellet particles. Solution analysis further demonstrated double hydrolysis of a PET oligomer, bis-(2-hydroxyethyl) terephthalate, to the bioavailable monomer terephthalate. Supplementation with acetate, a common wastewater co-substrate, promoted cell growth and PET fragmentation. Of the multiple hydrolases encoded in the genome, intracellular proteomics detected only one, which was found in both acetate-only and PET-only conditions. Homology modeling of this hydrolase structure illustrated substrate binding analogous to reported PET hydrolases despite dissimilar sequences. Mutants lacking this hydrolase gene were incapable of PET oligomer hydrolysis and had 25% decrease in PET fragmentation; re-insertion of the gene restored both functions. Thus, we have identified constitutive production of a key PET-degrading hydrolase in wastewater Comamonas, which can be exploited for plastics bioconversion.

Bio: Dr. Nanqing Zhou is a postdoctoral scholar working with Prof. Ludmilla Aristilde at the Department of Civil and Environmental Engineering at Northwestern University. Dr. Zhou obtained her B.S. in Environmental Science and M.S. in Environmental Engineering from the University of Science and Technology of China. She then obtained a doctoral degree in Marine Biosciences from the University of Delaware. Her research focuses on applying multi-omics approaches to unravel the metabolic network in biotechnologically relevant microbes using waste carbon sources.