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Sep27
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Title: Production of Nanoplastics and Bioavailable Carbons by Wastewater Comamonas
Abstract: Comamonadacae bacteria are enriched on polyethylene terephthalate (PET) microplastics in wastewaters and urban rivers, but the PET-degrading mechanisms remain unclear. Here, we investigated these mechanisms with Comamonas testosteroni KF-1, a wastewater isolate, by combining microscopy, spectroscopy, proteomics, protein modeling, and genetic engineering. Compared to minor dents on PET films, scanning electron microscopy revealed significant fragmentation of PET pellets, resulting in 3.5-fold increase in the abundance of small nanoparticles (< 100 nm) during 30-day cultivation. Infrared spectroscopy captured primarily hydrolytic cleavage in the fragmented pellet particles. Solution analysis further demonstrated double hydrolysis of a PET oligomer, bis-(2-hydroxyethyl) terephthalate, to the bioavailable monomer terephthalate. Supplementation with acetate, a common wastewater co-substrate, promoted cell growth and PET fragmentation. Of the multiple hydrolases encoded in the genome, intracellular proteomics detected only one, which was found in both acetate-only and PET-only conditions. Homology modeling of this hydrolase structure illustrated substrate binding analogous to reported PET hydrolases despite dissimilar sequences. Mutants lacking this hydrolase gene were incapable of PET oligomer hydrolysis and had 25% decrease in PET fragmentation; re-insertion of the gene restored both functions. Thus, we have identified constitutive production of a key PET-degrading hydrolase in wastewater Comamonas, which can be exploited for plastics bioconversion.
Bio: Dr. Nanqing Zhou is a postdoctoral scholar working with Prof. Ludmilla Aristilde at the Department of Civil and Environmental Engineering at Northwestern University. Dr. Zhou obtained her B.S. in Environmental Science and M.S. in Environmental Engineering from the University of Science and Technology of China. She then obtained a doctoral degree in Marine Biosciences from the University of Delaware. Her research focuses on applying multi-omics approaches to unravel the metabolic network in biotechnologically relevant microbes using waste carbon sources.
TIME Friday, September 27, 2024 at 2:00 PM - 3:00 PM
LOCATION A230, Technological Institute map it
CONTACT Andrew Liguori andrew.liguori@northwestern.edu EMAIL
CALENDAR McCormick - Civil and Environmental Engineering (CEE)
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Sep30
EVENT DETAILS
Last day to add a class or change a section for Fall (Last day for tuition adjustment related to enrollment changes (to or from full-time). No reductions are made to bills for dropped or swapped classes after this date)
TIME Monday, September 30, 2024
CONTACT Office of the Registrar nu-registrar@northwestern.edu EMAIL
CALENDAR University Academic Calendar
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Oct2
EVENT DETAILS
TBA
TIME Wednesday, October 2, 2024 at 1:00 PM - 2:00 PM
LOCATION Ford Hive Rm 2350, Ford Motor Company Engineering Design Center map it
CONTACT Andrew Liguori andrew.liguori@northwestern.edu EMAIL
CALENDAR McCormick - Civil and Environmental Engineering (CEE)
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Oct3
EVENT DETAILS
Department happy hour for students and faculty to get to know each other.
TIME Thursday, October 3, 2024 at 5:00 PM - 8:00 PM
LOCATION 750 Chicago Ave
CONTACT Andrew Liguori andrew.liguori@northwestern.edu EMAIL
CALENDAR McCormick - Civil and Environmental Engineering (CEE)
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Oct4
EVENT DETAILS
TBA
TIME Friday, October 4, 2024 at 2:00 PM - 3:00 PM
LOCATION A230, Technological Institute map it
CONTACT Andrew Liguori andrew.liguori@northwestern.edu EMAIL
CALENDAR McCormick - Civil and Environmental Engineering (CEE)
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Oct11
EVENT DETAILS
TBA
TIME Friday, October 11, 2024 at 2:00 PM - 3:00 PM
LOCATION A230, Technological Institute map it
CONTACT Andrew Liguori andrew.liguori@northwestern.edu EMAIL
CALENDAR McCormick - Civil and Environmental Engineering (CEE)
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Oct18
EVENT DETAILS
TBA
TIME Friday, October 18, 2024 at 2:00 PM - 3:00 PM
LOCATION A230, Technological Institute map it
CONTACT Andrew Liguori andrew.liguori@northwestern.edu EMAIL
CALENDAR McCormick - Civil and Environmental Engineering (CEE)
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Oct19
EVENT DETAILS
Annual CEE Fall Career Fair for students.
TIME Saturday, October 19, 2024 at 10:00 AM - 1:00 PM
LOCATION Louis Room, Norris University Center map it
CONTACT Andrew Liguori andrew.liguori@northwestern.edu EMAIL
CALENDAR McCormick - Civil and Environmental Engineering (CEE)
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Nov1
EVENT DETAILS
TBA
TIME Friday, November 1, 2024 at 2:00 PM - 3:00 PM
LOCATION A230, Technological Institute map it
CONTACT Andrew Liguori andrew.liguori@northwestern.edu EMAIL
CALENDAR McCormick - Civil and Environmental Engineering (CEE)
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Nov8
EVENT DETAILS
TBA
TIME Friday, November 8, 2024 at 2:00 PM - 3:00 PM
LOCATION A230, Technological Institute map it
CONTACT Andrew Liguori andrew.liguori@northwestern.edu EMAIL
CALENDAR McCormick - Civil and Environmental Engineering (CEE)
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Nov15
EVENT DETAILS
TBA
TIME Friday, November 15, 2024 at 2:00 PM - 3:00 PM
LOCATION A230, Technological Institute map it
CONTACT Andrew Liguori andrew.liguori@northwestern.edu EMAIL
CALENDAR McCormick - Civil and Environmental Engineering (CEE)
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Nov22
EVENT DETAILS
TBA
TIME Friday, November 22, 2024 at 2:00 PM - 3:00 PM
LOCATION A230, Technological Institute map it
CONTACT Andrew Liguori andrew.liguori@northwestern.edu EMAIL
CALENDAR McCormick - Civil and Environmental Engineering (CEE)